Waag Society is the first cultural institute wich obtained a S-I permit to give a GMO workshop. I was one of the 18 participants. During the workshop we will transform regular gut bacteria by introducing a gene from the bioluminescent jellyfish Aequorea victoria.
The pGLO plasmid is constructed with the jellyfish gene that encodes green fluorescent protein (GFP), an antibiotic-resistance gene that encodes β-lactamase protein, and the araC gene encoding a regulator protein that turns the GFP gene on and off. Bacteria transformed with pGLO plasmid are selected by ampicillin resistance; when induced to express GFP, the bugs glow fluorescent green under UV light.
The workshop takes place under strict procedures and follows the safety protocol.
Author- Nicole Spit
